Using the APEX-based proximity labeling technique, I want to assess how the loss of function of key ciliary proteins affects the ciliary proteome to better understand the molecular pathogenesis of ciliopathies.

Using ascorbate peroxidase-based proximity labeling I want to unravel the primary ciliary 'blue-print', which is needed to form this unique compartment.

On the trail of a newly identified ciliary protein with possible microtubule association, I am mainly focusing on basic biochemistry and microscopy approaches in cells and in vitro to characterize our "newcomer".

I am currently working with various biochemical methods to understand the function and transport of kinases and phosphatases in the primary cilium as well as their influence on the ciliary proteome and signalling pathways.

My aim is to study the role of primary cilia in differentiation processes of various cell types. I am especially interested in myogenesis and adipogenesis, as well as disease related cystogenesis.

In my project I am establishing new cilia-localized cAMP sensors to study cAMP dynamics in primary cilia.

I investigate how primary cilia signaling affects cell growth. 

Resident doctor | Internal medicine

Augusta Kliniken Bochum

Nicola established endogenous tagging approaches using the CRISPR/Cas12a system within the lab to help us study selected proteins in the cell’s more ‘natural’ state.

Shanli studied a so-far uncharacterized protein in primary cilia. This protein has recently been identified in one of our proteomic screens and she wanted to unravel its interactome in primary cilia.